Characterization, application and statistical optimization approach for enhanced production of pyocyanin pigment by Pseudomonas aeruginosa DN9

The aim of this work was to study the pyocyanin pigment from Pseudomonas aeruginosa DN9. involves optimization process parameters for enhanced production under submerged fermentation condition. During process, maximum (92.12 µg/ml) obtained with carbon source mannitole, nitrogen peptone, inorganic salt NaCl and metal ion FeSO4. Plackett Burman design Response Surface Methodology (RSM) showed KH2PO4 are significant variable in pigment. FTIR GC–MS done evaluate structural properties purified further analyzed as colouring agent inhibitory action against pathogenic microorganisms. Thus, present DN9 promising culture potential biotechnological application.